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UV-Vis Spectrophotometer

Nanotechnology Research Facility
Equipment
UV Vis Spectrophotometer

Specifications

  • Model: Varian Cary 50.
  • Monochromator: Czerny-Turner.
  • Beam splitting system: Beam splitter.
  • Detectors: 2 silicon diode detectors.
  • UV-Vis limiting resolution (nm): ≤ 1.5 nm.
  • Wavelength range (nm): 190--1100 nm.
  • Wavelength accuracy (nm): ± 0.5 at 541.94.

Principles

The principles of spectroscopic analysis rely on passing light of a known wavelength through a sample and measuring how much of the light is absorbed. (An alternative to this is to measure how much light is reflected.) A spectrophotometer must be able to generate discrete wavelengths of light, pass these through a sample and measure the absorption that has occurred.

Capabilities

  • Simple Reads module.
  • Scanning module.
  • Kinetics module.
  • Enzyme Kinetics module.
  • Scanning Kinetics module.
  • Instrument Validation module.

Applications

  • Quantitative and qualitative analysis.
  • Biosciences.
  • Pharmaceuticals.
  • Clinical Research and Forensics.
  • Food and Agriculture.
  • Chemical Analysis.
  • Environmental.
  • Fuels and Energy.
  • Material Sciences.